Identification and evaluation of resistance to sugarcane. Sugarcane streak mosaic is an important disease in sugarcane caused by sugarcane streak mosaic virus scsmv. Sugarcane cp31588 infected with scmvsc showing yellow streaks and slight. Augustinegrass virus symptoms splotchy, yellow streaking on leaf blades dead spots streaking on leaf blades unhealthy yellowish tinge andor dead turfgrass often excessive weeds december 5, 2014 master gardener hotline desk.
Here, we established a protocol of a onestep realtime quantitative reverse transcription pcr realtime qrtpcr using the taqman probe for the detection of scsmv in sugarcane. Detection and response of sugarcane against the infection of. The invention further provides a method for detecting the sugarcane streak mosaic virus. These symptoms included interveinal chlorotic specks, streaks or stripes on the leaves fig.
First occurrence of sugarcane streak mosaic virus infecting sugarcane in indonesia. Development of recombinant coat protein antibody based ic. Integrated disease management of sugarcane streak mosaic. According to the phylogenetic results of the complete genomic and coat protein gene sequenc. What you should know about sugarcane mosaic virus scmv. The virus was detected up to 125 and 1625 dilutions in infected sugarcane leaf, 5 l and 10 lwell in sugarcane juice, 125 and 125. These viruses do not occur in australia and are serious biosecurity threats to the australian sugarcane industry. Mosaic symptoms in sugarcane are currently associated with four diseases that are caused by several viruses rott et al.
Scsmv attack intensity at the sample location sugarcane crop may reach 62%. Identification of resistance to sugarcane streak mosaic. Sugarcane mosaic virus characterization of strains in brazil the sugarcane mosaic virus complex, in the genus potyvirus, family potyviridae, consists of seven viruses that infect different species of poaceous, and includes scmv, sorghum mosaic virus srmv, sugarcane streak mosaic virus scsmv, johnsongrass mosaic virus jgmv, maize. Researcharticle a onestep realtime rtpcr assay for the detection and quantitation of sugarcane streak mosaic virus weilinfu,1,2 shengrensun,1 huayingfu,1 ru. Cn102363819a primer for detecting sugarcane streak mosaic. Investigation on transmission modes and host range of. Jun 22, 2015 sugarcane mosaic disease is caused by the sugarcane streak mosaic virus scsmv. On the basis of the current taxonomic criteria, all isolates of potyviruses infecting sugarcane in australia have been found to be sugarcane mosaic virus. Bioekologi virus mosaik bergaris tebu sugarcane streak. It was first reported in north america in 1960 from ontario, canada. Distribution of sugarcane mosaic and sugarcane streak mosaic virus in india article in sugar tech 81. Virus mosaik bergaris tebu sugarcane streak mosaic virus memiliki partikel yang berbentuk batang lentur, filament dengan panjang 890 x 15 nm dengan satu bagian genom ssrna dengan panjang kirakira 10 kilobase kb.
A sugarcane plant which has recovered is not only symptomless, but the virus can no longer be detected in the. However, in louisiana, usa, most recent isolates are sorghum mosaic virus previously sugarcane mosaic virus strains h, i and m. Berat molekul dari protein mantel virus ini sebesar 40 kda lebih tinggi dari urutan asam amino. Virus mosaik bergaris tebu sugarcane streak mosaic virus merupakan salah satu penyakit penting yang menyebabkan mosaik pada tanaman tebu yang umumnya menyebar di negaranegara asia termasuk india dan indonesia, dan menyebabkan kehilangan hasil yang nyata. Distribution of sugarcane mosaic and sugarcane streak mosaic. A onestep realtime rtpcr assay for the detection and. The recombinant coat protein was used to raise high quality antiserum. The virus was first reported in 2005 and since then it has been widely distributed across java island. Particles were flexuous rods with an average length of 6678 nm and an approximate diameter of 1214 nm. On the other hand, mosaic symptoms caused by plant viruses such as sugarcane streak mosaic virus scsmv, sugarcane mosaic virus scmv, and sorghum mosaic virus srmv are dif. This video was developed for indonesian sugarcane growers and industry, to raise awareness of sugarcane streak mosaic virus, which does not exist in. Researcharticle a onestep realtime rtpcr assay for the detection and quantitation of sugarcane streak mosaic virus weilinfu,1,2 shengrensun,1 huayingfu,1 rukaichen,1 jinweisu,2 andsanjigao1,3. In other countries, mosaic diseases in sugarcane are caused by sorghum mosaic virus and sugarcane streak mosaic virus. Sugarcane streak mosaic virus scsmv is a pathogen only recognised in the late1990s.
Asia and widely infecting commercial crops, both in terms of incidence and in the percentage of plants infected within individual fields. Viral diseases affecting sugarcane include sugarcane mosaic virus, maize streak virus, and sugarcane yellow leaf virus. Seven sugarcane and sorghum leaf samples exhibiting mosaic symptoms and that originated from uttar pradesh, maharashtra and tamil nadu were characterized through rtpcr assays with specific designed primers forsugarcane mosaic virus, scmv f4 and r3, sugarcane streak mosaic virus, scsmvst2 and p1 andsorghum mosaic virus, srmv f3 and r3. Since scsmv is a new emerging virus the investigation on virus aetiology including transmission modes and virus host range is required. Some sugarcane varieties are capable of fixing atmospheric nitrogen in association with the bacterium gluconacetobacter diazotrophicus. Sugarcane mosaic disease is caused by the sugarcane streak mosaic virus scsmv. Scmv and srmv belong to potyvirus in the family potyviridae, while scsmv belongs to poacevirus of the family potyviridae. Sugarcane streak mosaic virus scsmv, causes mosaic disease of sugarcane and is thought to belong to a new undescribed genus in the family potyviridae. The virus, thus, was identified as a strain of sugarcane mosaic virus subbayya and raychaudhuri, 1970. Distribution, characterisation, yield losses and management approaches lilik koesmihartono putra, ari kristini. Research article a onestep realtime rtpcr assay for the.
Distribution of sugarcane mosaic and sugarcane streak. Seven sugarcane and sorghum leaf samples exhibiting mosaic symptoms and that originated from uttar pradesh, maharashtra and tamil nadu were characterized through rtpcr assays with specific designed primers forsugarcane mosaic virus, scmv f4 and r3,sugarcane streak mosaic virus, scsmvst2 and p1 andsorghum mosaic virus, srmv f3 and r3. Pdf sugarcane streak mosaic occurs in the south and southeast. At the end of 2017, the plant diagnostic clinic at uftrec discovered that some turfgrass samples from miamidade and monroe county tested positive for sugarcane mosaic virus.
Sugarcane mosaic virus scmv is a singlestranded, positivesense rna virus of the. Research article a onestep realtime rtpcr assay for the detection and quantitation of sugarcane streak mosaic virus weilinfu, 1,2 shengrensun, 1 huayingfu, 1 rukaichen, 1 jinweisu, 2 andsanjigao 1,3. Aug 25, 2018 sugarcane mosaic disease is one of the most serious and prevalent viral diseases of sugarcane in china. Sugarcane transcript profiling assessed by cdnaaflp. Pdf first occurrence of sugarcane streak mosaic virus. Sugarcane mosaic virus techne qpcr test 150 tests for general laboratory and research use only quantification of sugarcane mosaic virus genomes 1 advanced kit handbook hb10. It has been noted that some sugarcane plants recover from mosaic. In indonesian the research about sugarcane streak mosaic virus scsmv still not widely practiced, especially in the field. Jan 28, 2018 this video was developed for indonesian sugarcane growers and industry, to raise awareness of sugarcane streak mosaic virus, which does not exist in australia, but poses a risk to the australian. In sugarcane, this is the most widespread virus and 21 strains of it have been found in the united states.
The complete genomic sequence of sugarcane mosaic virus. Streak mosaiccaused by sugarcane streak mosaic virus scsmv. Sugarcane mosaic virus has a wide host range, including families of gramineae and potyviridae, and occurs in many regions of the world. First report of sugarcane streak mosaic virus in iran. Asia and widely infecting commercial crops, both in terms of incidence and in. Pdf streak mosaic is a new disease of sugarcane in indonesia caused by sugarcane streak mosaic virus scsmv. Sugarcane mosaic virus scmv is the prevalent virus inducing maize dwarf mosaic and sugarcane mosaic diseases in china.
Top of page there has been confusion over the taxonomy of maize streak virus msv with regard to synonyms and strains, mainly due to the geographical distribution and host range of the virus. Sugarcane mosaic disease is mainly caused by the sugarcane mosaic virus scmv, sorghum mosaic virus srmv and sugarcane streak mosaic virus scsmv. Jan 30, 2018 at the end of 2017, the plant diagnostic clinic at uftrec discovered that some turfgrass samples from miamidade and monroe county tested positive for sugarcane mosaic virus. Sugarcane streak mosaic virus, sugarcane yellow leaf virus, mixed infection, sugarcane introduction sugarcane is an important food cum cash crop and is the third largest crop in terms of value next to rice and wheat in india. The mosaic of sugarcane occurs in more than 70 countries and only scmv, srmv and scsmv can naturally infect this crop 3. Resistant varieties are the most economical and effective measures for controlling this disease.
Bowden, extension specialist, plant pathology wheat spindle streak mosaic virus wheat spindle streak mosaic virus wssmv is also known as wheat yellow mosaic virus. Symptoms were similar to another viral disease of st. Distribution, characterisation, yield losses and management approaches. Resistance against sugarcane streak mosaic virus scsmv and sorghum mosaic virus srmv in 41 excellent sugarcane innovation germplasms and their parents saccharum officinarum l. This is the first report on the molecular characterization of a virus causing mosaic disease of sugarcane in india and the results demonstrate that the virus is a strain of sugarcane streak mosaic.
Identification of resistance to sugarcane streak mosaic virus. Mild mosaic caused by sugarcane mild mosaic virus scmmv. The virus causes mosaic symptoms in sugarcane, maize, sorghum, and other poaceous plants. Research article a onestep realtime rtpcr assay for the detection and quantitation of sugarcane streak mosaic virus weilinfu, 1,2 shengrensun, 1 huayingfu, 1 rukaichen, 1 jinweisu, 2 andsanjigao 1,3 key laboratory of sugarcane biology and genetic breeding, ministry of agriculture, fujian agriculture and forestry university. Sugarcane mosaic virus an overview sciencedirect topics. Scmv, sorghum mosaic virus srmv, and sugarcane streak mosaic virus are the main pathogens that cause severe mosaic disease in sugarcane and result in heavy yield loss in the sugarcane industry. The coat protein cp gene from the andhra pradesh ap isolate of scsmv scsmv ap was cloned and expressed in escherichia coli. Zea mosaic virus zemv, belonging to the genus potyvirus, and sugarcane streak mosaic virus scsmv belonging to the new genus poace virus 1. The occurrence of sugarcane streak mosaic virus scsmv, characterized as susmovirus, a potyviridae member, was recently reported in india. Sugarcane streak mosaic virus wheat systemic silencing potyviridae poacevirus p1 hcpro inhibition abstract triticum mosaic virus trimv is the type species of the newly established poacevirus genus in the family potyviridae. Sugarcane mosaic virus characterization of strains in brazil the sugarcane mosaic virus complex, in the genus poty virus, family potyviridae, consists of seven viruses that infect different species of poaceous, and includes scmv, sorghum mosaic virus srmv, sugarcane streak mosaic virus scsmv, johnsongrass mosaic virus jgmv, maize. Notes on taxonomy and nomenclature top of page there has been confusion over the taxonomy of maize streak virus msv with regard to synonyms and strains, mainly due to the geographical distribution and host range of the virus.
It is susceptible to several biotic stresses in nature r ottet al. Identification of sugarcane host factors interacting with. The high intensity of the attack on the planting of sugar cane scsmv make the virus should receive serious attention in order not widespread dissemination. Symptoms mosaic disease is characterised by a mottled pattern. Sugarcane streak mosaic virus scsmv is a pathogenonly recognised in the late1990s hall. The complete genomic sequence of sugarcane mosaic virus from canna spp. Triticum mosaic poacevirus enlists p1 rather than hcpro. Farm sizes are small average around 1 ha and farming operations are almost exclusively undertaken by hand. Antibody and nucleic acid probebased techniques for. It was first found in kansas in sedgwick county in 1984, but it may. During september to november 2014, symptoms associated with scmv, sugarcane streak mosaic virus scsmv and sorghum mosaic virus infection were observed in some sugarcane fields.
Cn102363819a primer for detecting sugarcane streak. Virus mempunyai pengaruh yang beragam terhadap tanaman. Pdf molecular characterization of a new strain of sugarcane. Sugarcane streak mosaic scsm caused by sugarcane streak mosaic virus scsmv is a relatively recently recognised disease of sugarcane that is now widespread through s. General information about sugarcane streak mosaic virus scsmv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. On the other hand, mosaic symptoms caused by plant viruses such as sugarcane streak mosaic virus. Continuing to use this website means you agree to our use of cookies. Detection and response of sugarcane against the infection. Augustinegrass virus symptoms splotchy, yellow streaking on leaf blades dead spots streak ing on leaf blades unhealthy yellowish tinge andor dead turfgrass often excessive weeds december 5, 2014 master gardener hotline desk. Control of mosaic through heat treatment of cuttings is partially effective but is only practical in quarantine situations. Augustinegrass decline sad, caused by panicum mosaic virus, but as of november 2014, samples sent to the ufifas extension plant diagnostic center have tested negative for sad and positive for presence of sugarcane mosaic virus scmv.
The invention provides a primer for detecting sugarcane streak mosaic virus scsmv and a detection method thereof. Integrated disease management of sugarcane streak mosaic in. Mild mosaiccaused by sugarcane mild mosaic virus scmmv. Scsmv is a new pathogen that has been ascertained in recent years. Integrated sugarcane streak mosaic virus management. An overview of the sugarcane mosaic disease in south america. This disease has the potential to decrease the yield of sugarcane zahra, 20092. Sugarcane mosaic virus returns as threat to lawns miami a plant disease linked to sugarcane that had largely vanished 40 years ago has suddenly reappeared, becoming the first virus in the. Maize streak and maize mosaic viruses were most often found in central 4. Mixed infection of sugarcane streak mosaic and sugarcane. Streak mosaic caused by sugarcane streak mosaic virus scsmv. Augustinegrass virus symptoms splotchy, yellow streaking on leaves dead spots streaking on leaves unhealthy yellowish tinge andor dead lawn often excessive weeds february 2, 2017 master gardener hotline desk. Sugarcane mosaic disease is one of the most serious and prevalent viral diseases of sugarcane in china.